Highly sensitive transient absorption imaging of graphene and graphene oxide in living cells and circulating blood

We report a transient absorption (TA) imaging method for fast visualization and quantitative layer analysis of graphene and GO. Forward and backward imaging of graphene on various substrates under ambient condition was imaged with a speed of 2 μs per pixel. The TA intensity linearly increased with the layer number of graphene. Real-time TA imaging of GO in vitro with capability of quantitative analysis of intracellular concentration and ex vivo in circulating blood were demonstrated. These results suggest that TA microscopy is a valid tool for the study of graphene based materials

Optimization of a High-Speed X-Ray Imaging System for Studying Sprays

Spray-based liquid atomization and liquid mixing is critical for development of efficient combustors and drug delivery systems as well as multiple coating-related applications. While optical methods allow characterization of low density regions of sprays, the scattering of optical photons hinders the characterization of a dense core. Unlike optical photons, higher-energy X-ray photons have the capability to penetrate and image the core structure of sprays. Here we characterized temporal and spatial resolution of an X-Ray imaging system based on a commercially available tube source with an anode size of 0.6 mm. For high-speed imaging, a phosphor screen in combination with a high-speed CMOS camera equipped with a two-stage intensifier was used. Water was used as a model liquid with the addition of potassium iodide to increase the X-Ray absorption coefficient. Two-dimensional images of 0.5mm and 2 mm impinging jet sprays were taken with differing spatial resolutions and potassium iodide mass concentrations. Depending on the spray conditions, optimal imaging settings were found. The technique can be extended to three-dimensional analysis of sprays with multiple viewing angles from two or more X-ray sources along with tomographic reconstruction

Intense pulsed helium droplet beams

Pulsed (30 - 100 microseconds) nozzle beams have been used to generate helium droplets ( = 10^4-10^5). The dependence of the beam intensity and the mean droplet size on the source stagnation pressure and temperature are studied via mass spectroscopy and laser induced fluorescence of embedded phthalocyanine molecules. In comparison to a cw beam the pulsed source for the same pressure and temperature has a factor of 100 higher flux and the droplet sizes are an order of a magnitude larger.Comment: Accepted by Review of Scientific Instrument

Biaxial deformation of collagen and elastin fibers in coronary adventitia

The microstructural deformation-mechanical loading relation of the blood vessel wall is essential for understanding the overall mechanical behavior of vascular tissue in health and disease. We employed simultaneous mechanical loading-imaging to quantify in situ deformation of individual collagen and elastin fibers on unstained fresh porcine coronary adventitia under a combination of vessel inflation and axial extension loading. Specifically, the specimens were imaged under biaxial loads to study microscopic deformation-loading behavior of fibers in conjunction with morphometric measurements at the zero-stress state. Collagen fibers largely orientate in the longitudinal direction, while elastin fibers have major orientation parallel to collagen, but with additional orientation angles in each sublayer of the adventitia. With an increase of biaxial load, collagen fibers were uniformly stretched to the loading direction, while elastin fibers gradually formed a network in sublayers, which strongly depended on the initial arrangement. The waviness of collagen decreased more rapidly at a circumferential stretch ratio of λθ = 1.0 than at λθ = 1.5, while most collagen became straightened at λθ = 1.8. These microscopic deformations imply that the longitudinally stiffer adventitia is a direct result of initial fiber alignment, and the overall mechanical behavior of the tissue is highly dependent on the corresponding microscopic deformation of fibers. The microstructural deformation-loading relation will serve as a foundation for micromechanical models of the vessel wall